Local landowners on Manus Island in PNG have blocked access to the Navy base where the Manus Island immigration detention centre is.
They have also stopped the Australian-run centre from using the local rubbish dump.
The protest comes amid anger from locals who say they are being blocked from providing services and labour to the centre, despite commitments to the contrary.
The MP for Manus Island, Ronny Knight, says he wants the centre at the Lombrum Navy base closed until the end of Australia's federal election.
He says local businesses are being shut out and claims residents wanting to work there are being paid less than workers brought in from elsewhere.
Mr Knight says that in response, landowners are taking the matter into their own hands.
"The landowners have closed down the dump at Lombrum, and they're about to close down the gate," he said.
"That's the main gate leading into the base."
Meanwhile another 40 men have been sent to Manus Island under the Government's resettlement deal struck last month.
All asylum seekers arriving in Australia by boat are now being processed and resettled in Papua New Guinea.
The latest group is the 12th to be sent under the deal and comprises 38 Iranians and two Iraqis.
Mr Knight says he supported the reopening of the Manus Island centre, saying at the time it came with a commitment that locals would benefit.