Clive Palmer says ex-military officers are working for the electoral commission and may be part of a conspiracy to deny him victory in the seat of Fairfax.
Mr Palmer says at least 10 former military members have central positions in the Australian Electoral Commission (AEC).
"It's a bad thing because those people have links to the military and they have a network and camaraderie within the AEC because they have a common background," he told AAP.
"I think they may give orders to people in the system.
"The AEC should employ people who are independent."
The billionaire candidate wants the AEC to give him the names of people who signed ballots in Fairfax and specimens of their initials.
He said many ballot papers hadn't been initialled and suggested the ballot boxes had been tampered with.
"Now I'm not saying all electoral officers are crooks but if there is nothing to hide, then why won't they give us the names of people who initialled them?" he said.
Mr Palmer made the comments as counting showed his LNP opponent Ted O'Brien was eating away at his lead in the Sunshine Coast seat of Fairfax.
He is in front by 1132 votes, down from 1440 on Wednesday night.
On Tuesday, Mr Palmer predicted he would lose Fairfax because of unfair electoral processes, including voting irregularities and ballot security.
He has threatened to have the Palmer United Party's two Senate bound candidates block legislation unless electoral reform is promised.
AEC spokeswoman Anne Bright said she was unable to comment on any specific allegations.
Any petition lodged needed to be evidence-based, she said.