They had known for months that it was coming but confirmation that nearly 60 vision impaired workers would lose their jobs was a devastating blow.
There were tears as workers were told in the canteen of Vision Australia Enterprises' Melbourne workshop on Thursday that it would be closing.
The organisation's Brisbane workshop will also shut down, while three workers who provide braille library services in Sydney will lose their jobs.
In total 71 people will lose their jobs at the three sites, with 58 vision impaired workers among them.
"The people here are distressed, traumatised," Blind Workers Union vice president Martin Stewart told AAP.
"Quite frankly they feel extremely abandoned and it's true, they have been abandoned."
Mr Stewart said there was virtually no prospect of the workers finding another job in the manual labour area.
The decision was first announced in August and workers rallied in Melbourne and Brisbane hoping it would be reversed.
Vision Australia chief executive Ron Hooton said the organisation had struggled for 15 years to make operations viable.
"We've done everything we possibly can," he told ABC Radio on Thursday.
Mr Hooton said disability enterprises in Australia were struggling to stay afloat.
"Vision Australia must focus on what it's good at and where we can get the best benefit for the low vision community," he said.
Mr Hooton said alternative positions would be sought for all workers who had lost jobs and 20 positions would be made available internally.